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Image Search Results
Journal: bioRxiv
Article Title: Cancer-specific sialylation of insulin-like growth factor 1 receptor impairs therapeutic antibody binding and efficacy
doi: 10.1101/2025.10.17.682592
Figure Lengend Snippet: (a) Western Blot of IGF1R alpha after SNA pulldown in ST6GAL1 knockout cells (b) Western blot of IGF1R alpha immunoprecipitated with ganitumab in ST6GAL1 knockout cells (c) Western blot of p-IGF1R in ST6GAL1 knockout cells treated with ganitumab (d) ST6GAL1 expression in ovarian cancer tumor vs normal ovary tissue from TCGA data analyzed by Xenabrowser. (e) Ganitumab H-score of ovarian cancer FFPE tissues stratified into ST6GAL1-High (n=12) and ST6GAL1-Low (n=12) after sialidase (SIA) digestion; representative image of the staining and H&E staining of the tissues shown at 40x magnification. Scale bar = 50μm.
Article Snippet: The antibodies and lectin used include: IGF1R α (Santa Cruz, Catalog# sc-271606), IGF1R β (Cell Signaling Technology, Catalog# 3027), phospho-IGF1R β (Cell Signaling Technology, Catalog# 4568),
Techniques: Western Blot, Knock-Out, Immunoprecipitation, Expressing, Staining